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Research
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Publications
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All publications
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Benner, SA
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Carrigan, MA
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Chamberlin, SG
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Chen, F
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Hughes, E
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Hutter, D
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Hoshika, S
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Karalkar, N
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Kim, HJ
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Kim, MJ
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Laos, R
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Leal, NA
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Lyons, TJ
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Moussatche, P
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Shaw, RW
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Yang, ZY
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People
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Benner, Steven
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Carrigan, Matthew
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Chamberlin, Steve
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Chen, Fei
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Davis, Ross
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Hoshika, Shuichi
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Hughes, Ewa
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Hughes, Romaine
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Hutter, Daniel
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Karalkar, Nilesh
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Kim, Hyo-Joong
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Kim, Myong
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Laos, Roberto
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Leal, Nicole
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Lyons, Thomas
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Moussatche, Patricia
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Opalko, Jeff
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Shaw, Ryan
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Yang, Zunyi
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Associate Fei Chen
Education
- BS in Microbiology. Sichuan University, China
(1995)
- PhD in Biochemistry and Molecular Biology. Jilin University, China
(2003)
- Postdoctoral Research Associate. University of Florida
(2005)
Research summary
My research focuses on the development of a novel self-avoiding
molecular recognition system (SAMRS) for the amplification of gene
fragments in genome. The components of a SAMRS do bind to natural DNA
or RNA, but do not bind to other components of the same unnatural
SAMRS system, which is achieved by different hydrogen bonding patterns
of base pairs.
Publications
 A novel replicating circular DNAzyme
Chen, F
Wang, RJ
Li, Z
Liu, B
Wang, XP
Sun, YH
Hao, DY
Zhang, J
Nucl. Acids Res. 32
(8)
2336-2341
(2004)
<Abstract>
10-23 DNAzyme has the potential to suppress gene expressions through
sequence-specific mRNA cleavage. However, the dependence on exogenous
delivery limits its applications. The objective of this work is to
establish a replicating DNAzyme in bacteria using a single-stranded DNA
vector. By cloning the 10-23 DNAzyme into the M13mp18 vector, we
constructed two circular DNAzymes, C-Dz(7) and C-Dz(482), targeting the
beta-lactamase mRNA. These circular DNAzymes showed in vitro catalytic
efficiencies (k(cat)/K-M) of 7.82 x 10(6) and 1.36 x 10(7) M-1.min(-1),
respectively. Their dependence on divalent metal ions is similar to
that found with linear 10-23 DNAzyme. Importantly, the circular
DNAzymes were not only capable of replicating in bacteria but also
exhibited high activities in inhibiting beta-lactamase and bacterial
growth. This study thus provides a novel strategy to produce
replicating DNAzymes which may find widespread applications.
Inhibition of ampicillin-resistant bacteria by novel mono-DNAzymes and di-DNAzyme targeted to beta-lactamase mRNA
Chen, F
Li, Z
Wang, RJ
Liu, B
Zeng, Z
Zhang, HY
Zhang, J
Oligonucleotides 14
(2)
80-89
(2004)
<Abstract>
In view of the weakness of antibiotics and the properties of antisense
drugs, we applied DNAzymes to the field of drug resistance in bacteria.
Two 10-23 mono-DNAzymes (Dz(1), Dz(2)) and a di-DNAzyme (Dz(1-2))
targeted to beta-lactamase mRNA were designed to determine to what
degree the growth of ampicillin-resistant bacteria (TEM-1, TEM-3) was
inhibited. All three DNAzymes can play a role both in vitro and in
vivo. In vitro, they exhibited high catalytic efficiency (k(cat)/K-M)
of 63.5, 91.1, and 30.8 pM(-1) (.) min(-1), respectively, under
multiple-turnover conditions. In vivo, after 9 hours' incubation, the
degree of inhibition of Dz(1), Dz(2), and Dz(1-2) for TEM-1 bacteria
was 27.2%, 39.6%, and 57.7%, respectively, and that for TEM-3 bacteria
was 39.1%, 44%, and 62.6%, respectively. Dz(1-2) showed the greatest
inhibiting effect, demonstrating in vivo activity may be increased by
constructing multiple-target DNAzymes. The results indicated a
potential possibility for DNAzymes to act as a new type of
antibacterial or a tool of gene functional analysis for prokaryocytes.
PTC effect of carbon black filled polypropylene
He, XJ
Chen, F
Chen, XF
J. Mater. Sci. Lett. 20
(7)
589-590
(2001)
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