FFAME.org :: Fei Chen's Publications

Expanded Genetic Alphabets in the Polymerase Chain Reaction
Yang, ZY Chen, F Chamberlin, SG Benner, SA
Angew. Chem. Int. Ed. 49 (1) 177-180 (2010)

Artificial Genetic Systems: Self-Avoiding DNA in PCR and Multiplexed PCR
Hoshika, S Chen, F Leal, NA Benner, SA
Angew. Chem. Int. Ed. 49 (32) 5554-5557 (2010)

Reconstructed evolutionary adaptive paths give polymerases accepting reversible terminators for sequencing and SNP detection
Chen, F Gaucher, EA Leal, NA Hutter, D Havemann, SA Govindarajan, S Ortlund, EA Benner, SA
Proc. Natl. Acad. Sci. USA 107 (5) 1948-1953 (2010)

Self-Avoiding Molecular Recognition Systems (SAMRS)
Hoshika, S Chen, F Leal, NA Benner, SA
Nucleic Acids Symp. Ser. 52 (1) 129-130 (2008)

A novel replicating circular DNAzyme
Chen, F Wang, RJ Li, Z Liu, B Wang, XP Sun, YH Hao, DY Zhang, J
Nucl. Acids Res. 32 (8) 2336-2341 (2004)
<Abstract>

10-23 DNAzyme has the potential to suppress gene expressions through sequence-specific mRNA cleavage. However, the dependence on exogenous delivery limits its applications. The objective of this work is to establish a replicating DNAzyme in bacteria using a single-stranded DNA vector. By cloning the 10-23 DNAzyme into the M13mp18 vector, we constructed two circular DNAzymes, C-Dz(7) and C-Dz(482), targeting the beta-lactamase mRNA. These circular DNAzymes showed in vitro catalytic efficiencies (k(cat)/K-M) of 7.82 x 10(6) and 1.36 x 10(7) M-1.min(-1), respectively. Their dependence on divalent metal ions is similar to that found with linear 10-23 DNAzyme. Importantly, the circular DNAzymes were not only capable of replicating in bacteria but also exhibited high activities in inhibiting beta-lactamase and bacterial growth. This study thus provides a novel strategy to produce replicating DNAzymes which may find widespread applications.

Inhibition of ampicillin-resistant bacteria by novel mono-DNAzymes and di-DNAzyme targeted to beta-lactamase mRNA
Chen, F Li, Z Wang, RJ Liu, B Zeng, Z Zhang, HY Zhang, J
Oligonucleotides 14 (2) 80-89 (2004)
<Abstract>

In view of the weakness of antibiotics and the properties of antisense drugs, we applied DNAzymes to the field of drug resistance in bacteria. Two 10-23 mono-DNAzymes (Dz(1), Dz(2)) and a di-DNAzyme (Dz(1-2)) targeted to beta-lactamase mRNA were designed to determine to what degree the growth of ampicillin-resistant bacteria (TEM-1, TEM-3) was inhibited. All three DNAzymes can play a role both in vitro and in vivo. In vitro, they exhibited high catalytic efficiency (k(cat)/K-M) of 63.5, 91.1, and 30.8 pM(-1) (.) min(-1), respectively, under multiple-turnover conditions. In vivo, after 9 hours' incubation, the degree of inhibition of Dz(1), Dz(2), and Dz(1-2) for TEM-1 bacteria was 27.2%, 39.6%, and 57.7%, respectively, and that for TEM-3 bacteria was 39.1%, 44%, and 62.6%, respectively. Dz(1-2) showed the greatest inhibiting effect, demonstrating in vivo activity may be increased by constructing multiple-target DNAzymes. The results indicated a potential possibility for DNAzymes to act as a new type of antibacterial or a tool of gene functional analysis for prokaryocytes.

PTC effect of carbon black filled polypropylene
He, XJ Chen, F Chen, XF
J. Mater. Sci. Lett. 20 (7) 589-590 (2001)